SP2 CRISPR/Cas-vermitteltes Genome editing zur Untersuchung der Prozesse der Leberentzündung und ihrer Konsequenzen

Z2_April 2014


Prof. Dr. rer. nat. Boris Fehse
Institut für Zell- und Gentherapie
Klinik für Stammzelltransplantation
Universitätsklinikum Hamburg-Eppendorf
Martinistrasse 52
20246 Hamburg
Tel.: +49-40-7410-55518
Fax: +49-40-7410-55468
E-Mail: fehse@uke.uni-hamburg.de

Prof. Eithan Galun, MD
Goldyne Savad Institute of Gene Therapy
Hadassah Medical Center
The Hebrew University of Jerusalem
Jerusalem, 91120
Tel.: +972-2-6778589; +972-2-6777762
Fax: +972-2-6430982
E-mail: eithang@hadassah.org.il


Functional gene analysis is pivotal for a better understanding of physiologic as well as patho-logic processes. To address gene functions, over-expression and/or down-regulation studies can be performed.

Efficient technologies for transient as well as permanent gene transfer are essential tools to perform gene-function studies. In addition, genome editing facilitates permanent gene knock-out.

SP2 in 60 SekundenVideoarchiv

online seit 04.11.2012

In the first support period, SP2 has provided partners of SFB841 with a broad range of ”state-of-the-art“ technologies for the permanent and transient gene transfer into liver as well as im-mune cells. Different vectors, e.g. based on our LeGO platform, have been designed and cloned in accordance with individual project needs of the SFB partners.

In the second period of support, a particular focus will be laid on the provision of methods for the efficient delivery of TALE nucleases facilitating site-specific and permanent gene disruption to allow loss-of-function and gain-of-function studies in cell lines as well as in primary cells.

Aktuelles aus der Arbeitsgruppe:

Chai C, Rivkin M, Berkovits L, Simerzin A, Zorde-Khvalevsky E, Rosenberg N, Klein S, Yaish D, Durst R, Shpitzen S, Udi S, Tam J, Heeren J, Worthmann A, Schramm C, Kluwe J, Ravid R, Hornstein E, Giladi H, Galun E.

Metabolic Circuit Involving Free Fatty Acids, microRNA 122, and Triglyceride Synthesis in Liver and Muscle Tissues
Gastroenterology. 2017 Aug 9. pii: S0016-5085(17)36024-9. doi:10.1053/j.gastro.2017.08.013. [Epub ahead of print]

Lanton T, Shriki A, Nechemia-Arbely Y, Abramovitch R, Levkovitch O, Adar R, Rosenberg N, Paldor M, Goldenberg D, Sonnenblick A, Peled A, Rose John S, Galun E, Axelrod JH

Interleukin 6-dependent genomic instability heralds accelerated carcinogenesis following liver regeneration on a background of chronic hepatitis
Hepatology. 2017 May;65(5):1600-1611. doi: 10.1002/hep.29004. Epub 2017 Mar 23

Mohme M, Maire CL, Riecken K, Zapf S, Aranyossy T, Westphal M, Lamszus K, Fehse B

Optical Barcoding for Single-Clone Tracking to Study Tumor Heterogeneity
Mol Ther. 2017 Mar 1;25(3):621-633. doi: 10.1016/j.ymthe.2016.12.014. Epub 2017 Jan 18

Mock U, Hauber I, Fehse B

Digital PCR to assess gene-editing frequencies (GEF-dPCR) mediated by designer nucleases
Nat Protoc. 2016 Mar;11(3):598-615. doi: 10.1038/nprot.2016.027. Epub 2016 Feb 25.

Rivkin M, Simerzin A, Zorde-Khvalevsky E, Chai C, Yuval JB, Rosenberg N, Harari-Steinfeld R, Schneider R, Amir G, Condiotti R, Heikenwalder M, Weber A, Schramm C, Wege H, Kluwe J, Galun E, Giladi H

Inflammation-Induced Expression and Secretion of MicroRNA 122 Leads to Reduced Blood Levels of Kidney-derived Erythropoietin and Anemia
Gastroenterology. 2016 Jul 28. pii:S0016-5085(16)34827-2. doi: 10.1053/j.gastro.2016.07.031. Epub 2016 Jul 29