SP2 - Optimised tools for TALEN gene transfer into liver and immune cells
Prof. Dr. rer. nat. Boris Fehse
Institut für Zell- und Gentherapie
Klinik für Stammzelltransplantation
Prof. Eithan Galun, MD
Goldyne Savad Institute of Gene Therapy
Hadassah Medical Center
The Hebrew University of Jerusalem
Tel.: +972-2-6778589; +972-2-6777762
Functional gene analysis is pivotal for a better understanding of physiologic as well as patho-logic processes. To address gene functions, over-expression and/or down-regulation studies can be performed.
Efficient technologies for transient as well as permanent gene transfer are essential tools to perform gene-function studies. In addition, genome editing facilitates permanent gene knock-out.
SP2 in 60 SekundenVideoarchivonline seit 04.11.2012
In the first support period, SP2 has provided partners of SFB841 with a broad range of ”state-of-the-art“ technologies for the permanent and transient gene transfer into liver as well as im-mune cells. Different vectors, e.g. based on our LeGO platform, have been designed and cloned in accordance with individual project needs of the SFB partners.
In the second period of support, a particular focus will be laid on the provision of methods for the efficient delivery of TALE nucleases facilitating site-specific and permanent gene disruption to allow loss-of-function and gain-of-function studies in cell lines as well as in primary cells.
Aktuelles aus der Arbeitsgruppe:
Mock U, Hauber I, Fehse B
Digital PCR to assess gene-editing frequencies (GEF-dPCR) mediated by designer nucleases
Nat Protoc. 2016 Mar;11(3):598-615. doi: 10.1038/nprot.2016.027. Epub 2016 Feb 25.
Gomez-Nicola D, Riecken K, Perry VH, Fehse B
Multicolor RGB Marking Allows Morphometric and Functional Analysis of Hippocampal Granule Neurons at the Single-Cell Level
Hum Gene Ther. 2015 Jun;26(6):332-3. doi: 10.1089/hum.2015.010. Epub 2015 Apr 20.