Z2 - CRISPR/Cas-mediated genome editing for the study of processes involved in liver inflammation and its consequences

Z2_April 2014

Principal Investigators:
Prof. Dr. rer. nat. Boris Fehse
Institut für Zell- und Gentherapie
Klinik für Stammzelltransplantation
Universitätsklinikum Hamburg-Eppendorf
Martinistrasse 52
20246 Hamburg
Tel.: +49-40-7410-55518
Fax: +49-40-7410-55468
E-Mail: fehse@uke.uni-hamburg.de

Prof. Eithan Galun, MD
Goldyne Savad Institute of Gene Therapy
Hadassah Medical Center
The Hebrew University of Jerusalem
Jerusalem, 91120
Israel
Tel.: +972-2-6778589; +972-2-6777762
Fax: +972-2-6430982
E-mail: eithang@hadassah.org.il

Participating staff:

Dr. Kristoffer Riecken – PostDoc
Dr. Dawid Glow – PostDoc
Hilla Giladi, Ph.D. – PostDoc
Almut Uhde – technical assistant

Project description
Functional gene analysis, particularly based on genome editing, will be crucial to study the role of individual genes and gene networks in all different phases of liver inflam-mation and its consequences.

This service project will support members of CRC 841 in (i) performing CRISPR/Cas-based screening approaches, (ii) generating gene-edited cell lines, (iii) establishing (germ-line as well as liver-specific) “CRISPRed” mice, and (iv) permanent vector-based transgenesis.

SP2 in 60 SecondsVideoarchiv

online seit 02.10.2018

In the first support period, SP2 has provided partners of SFB841 with a broad range of ”state-of-the-art“ technologies for the permanent and transient gene transfer into liver as well as im-mune cells. Different vectors, e.g. based on our LeGO platform, have been designed and cloned in accordance with individual project needs of the SFB partners.

In the second period of support, a particular focus will be laid on the provision of methods for the efficient delivery of TALE nucleases facilitating site-specific and permanent gene disrup-tion to allow loss-of-function and gain-of-function studies in cell lines as well as in primary cells.

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Clonal dynamics studied in cultured iPS cells reveal major growth imbalances within few weeks.
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