SP2 CRISPR/Cas-vermitteltes Genome editing zur Untersuchung der Prozesse der Leberentzündung und ihrer Konsequenzen

SP2_Fehse_Galun_FP3

Leiter:

Prof. Dr. rer. nat. Boris Fehse
Institut für Zell- und Gentherapie
Klinik für Stammzelltransplantation
Universitätsklinikum Hamburg-Eppendorf
Martinistrasse 52
20246 Hamburg
Tel.: +49-40-7410-55518
Fax: +49-40-7410-55468
E-Mail: fehse@uke.uni-hamburg.de

Prof. Eithan Galun, MD
Goldyne Savad Institute of Gene Therapy
Hadassah Medical Center
The Hebrew University of Jerusalem
Jerusalem, 91120
Israel
Tel.: +972-2-6778589; +972-2-6777762
Fax: +972-2-6430982
E-mail: eithang@hadassah.org.il

Weitere beteiligte Mitarbeiter:

Dr. Kristoffer Riecken – PostDoc
Dr. Dawid Glow – PostDoc
Hilla Giladi, Ph.D. – PostDoc
Almut Uhde – Technische Assistentin

SP2 in 60 SekundenVideoarchiv

SP2 in 60 Sekunden online seit 02.10.2018

Aktuelles aus der Arbeitsgruppe:

Mirow M, Schwarze LI, Fehse B, Riecken K.

Efficient Pseudotyping of Different Retroviral Vectors Using a Novel, Codon-Optimized Gene for Chimeric GALV Envelope.
Viruses. 2021; 13(8):1471. doi: 10.3390/v13081471.

Schwarze LI, Głów D, Sonntag T, Uhde A, Fehse B.

Optimisation of a TALE nuclease targeting the HIV co-receptor CCR5 for clinical application.
Gene Ther. 2021 Jun 11. doi: 10.1038/s41434-021-00271-9. Epub ahead of print.

Głów D, Meyer S, Roldán IG, Akingunsade LM, Riecken K, Fehse B.

LATE – a novel sensitive cell-based assay for the study of CRISPR/Cas9 related Long-term Adverse Treatment Effects.
Molecular Therapy: Methods & Clinical Development, 2021. doi: https://doi.org/10.1016/j.omtm.2021.07.004.

Kempski J, Giannou AD, Riecken K, Zhao L, Steglich B, Lücke J, Garcia-Perez L, Karstens KF, Wöstemeier A, Nawrocki M, Pelczar P, Witkowski M, Nilsson S, Konczalla L, Shiri AM, Kempska J, Wahib R, Brockmann L, Huber P, Gnirck AC, Turner JE, Zazara DE, Arck PC, Stein A, Simon R, Daubmann A, Meiners J, Perez D, Strowig T, Koni P, Kruglov AA, Sauter G, Izbicki JR, Guse AH, Rösch T, Lohse AW, Flavell RA, Gagliani N, Huber S.

IL22BP Mediates the Antitumor Effects of Lymphotoxin Against Colorectal Tumors in Mice and Humans.
Gastroenterology. 2020 Oct;159(4):1417-1430.e3. doi: 10.1053/j.gastro.2020.06.033. Epub 2020 Jun 22.

Maire CL, Mohme M, Bockmayr M, Fita KD, Riecken K, Börnigen D, Alawi M, Failla A, Kolbe K, Zapf S, Holz M, Neumann K, Dührsen L, Lange T, Fehse B, Westphal M, Lamszus K.

Glioma escape signature and clonal development under immune pressure.
J Clin Invest. 2020 Oct 1;130(10):5257-5271. doi: 10.1172/JCI138760.

Mashel TV, Tarakanchikova YV, Muslimov AR, Zyuzin MV, Timin AS, Lepik KV, Fehse B.

Overcoming the delivery problem for therapeutic genome editing: Current status and perspective of non-viral methods.
Biomaterials. 2020 Aug 6;258:120282. doi: 10.1016/j.biomaterials.2020.120282. [Epub ahead of print].

Brenière-Letuffe D, Domke-Shibamiya A, Hansen A, Eschenhagen T, Fehse B, Riecken K, Stenzig J

Clonal dynamics studied in cultured iPS cells reveal major growth imbalances within few weeks.
Stem Cell Research & Therapy. 2018 in press.

Timin AS, Muslimov AR, Lepik KV, Epifanovskaya OS, Shakirova AI, Mock U, Riecken K, Okilova MV, Sergeev VS, Afanasyev BV, Fehse B, Sukhorukov GB

Efficient gene editing via non-viral delivery of CRISPR-Cas9 system using polymeric and hybrid microcarriers.
Nanomedicine. 2018 Jan;14(1):97-108. doi: 10.1016/j.nano.2017.09.001. Epub 2017 Sep 14.

Giersch K, Bhadra OD, Volz T, Allweiss L, Riecken K, Fehse B, Lohse AW, Petersen J, Sureau C, Urban S, Dandri M, Lütgehetmann M.

Hepatitis delta Virus persists during liver regeneration and is amplified through cell division both in vitro and in vivo.
Gut. 2017 Dec 7. pii: gutjnl-2017-314713. doi:10.1136/gutjnl-2017-314713. [Epub ahead of print]

Chai C, Rivkin M, Berkovits L, Simerzin A, Zorde-Khvalevsky E, Rosenberg N, Klein S, Yaish D, Durst R, Shpitzen S, Udi S, Tam J, Heeren J, Worthmann A, Schramm C, Kluwe J, Ravid R, Hornstein E, Giladi H, Galun E.

Metabolic Circuit Involving Free Fatty Acids, microRNA 122, and Triglyceride Synthesis in Liver and Muscle Tissues
Gastroenterology. 2017 Aug 9. pii: S0016-5085(17)36024-9. doi:10.1053/j.gastro.2017.08.013. [Epub ahead of print]

Lanton T, Shriki A, Nechemia-Arbely Y, Abramovitch R, Levkovitch O, Adar R, Rosenberg N, Paldor M, Goldenberg D, Sonnenblick A, Peled A, Rose John S, Galun E, Axelrod JH

Interleukin 6-dependent genomic instability heralds accelerated carcinogenesis following liver regeneration on a background of chronic hepatitis
Hepatology. 2017 May;65(5):1600-1611. doi: 10.1002/hep.29004. Epub 2017 Mar 23

Mohme M, Maire CL, Riecken K, Zapf S, Aranyossy T, Westphal M, Lamszus K, Fehse B

Optical Barcoding for Single-Clone Tracking to Study Tumor Heterogeneity
Mol Ther. 2017 Mar 1;25(3):621-633. doi: 10.1016/j.ymthe.2016.12.014. Epub 2017 Jan 18

Projektbeschreibung

Funktionelle Genanalysen, insbesondere basierend auf dem CRISPR/Cas-System, stellen eine essentielle Methodik zur Aufdeckung der Rolle einzelner Gene wie auch von Gennetzwerken im Zuge der Leberentzündung und ihrer Konsequenzen dar.

Dieses Serviceprojekt wird die Partner des SFB 841 bei folgenden Anwendungen unter-stützen: (i) CRISPR/Cas-basierte Screenings, (ii) Herstellung geneditierter Zelllinien, (iii) Etablierung (Keimbahn wie auch leberspezifischer) „geCRISPRter” Mäuse, (iv) permanente vektorvermittelte Transgenese.

In the first support period, SP2 has provided partners of SFB841 with a broad range of ”state-of-the-art“ technologies for the permanent and transient gene transfer into liver as well as im-mune cells. Different vectors, e.g. based on our LeGO platform, have been designed and cloned in accordance with individual project needs of the SFB partners.

In the second period of support, a particular focus will be laid on the provision of methods for the efficient delivery of TALE nucleases facilitating site-specific and permanent gene disruption to allow loss-of-function and gain-of-function studies in cell lines as well as in primary cells.