Z2 - CRISPR/Cas-mediated genome editing for the study of processes involved in liver inflammation and its consequences

Z2_April 2014

Principal Investigators:
Prof. Dr. rer. nat. Boris Fehse
Institut für Zell- und Gentherapie
Klinik für Stammzelltransplantation
Universitätsklinikum Hamburg-Eppendorf
Martinistrasse 52
20246 Hamburg
Tel.: +49-40-7410-55518
Fax: +49-40-7410-55468
E-Mail: fehse@uke.uni-hamburg.de

Prof. Eithan Galun, MD
Goldyne Savad Institute of Gene Therapy
Hadassah Medical Center
The Hebrew University of Jerusalem
Jerusalem, 91120
Tel.: +972-2-6778589; +972-2-6777762
Fax: +972-2-6430982
E-mail: eithang@hadassah.org.il

Participating staff:

Dr. Kristoffer Riecken – PostDoc
Dr. Dawid Glow – PostDoc
Hilla Giladi, Ph.D. – PostDoc
Almut Uhde – technical assistant

Project description
Functional gene analysis, particularly based on genome editing, will be crucial to study the role of individual genes and gene networks in all different phases of liver inflam-mation and its consequences.

This service project will support members of CRC 841 in (i) performing CRISPR/Cas-based screening approaches, (ii) generating gene-edited cell lines, (iii) establishing (germ-line as well as liver-specific) “CRISPRed” mice, and (iv) permanent vector-based transgenesis.

SP2 in 60 SecondsVideoarchiv

SP2 in 60 Seconds online seit 02.10.2018

In the first support period, SP2 has provided partners of SFB841 with a broad range of ”state-of-the-art“ technologies for the permanent and transient gene transfer into liver as well as im-mune cells. Different vectors, e.g. based on our LeGO platform, have been designed and cloned in accordance with individual project needs of the SFB partners.

In the second period of support, a particular focus will be laid on the provision of methods for the efficient delivery of TALE nucleases facilitating site-specific and permanent gene disrup-tion to allow loss-of-function and gain-of-function studies in cell lines as well as in primary cells.

Related News:

Brenière-Letuffe D, Domke-Shibamiya A, Hansen A, Eschenhagen T, Fehse B, Riecken K, Stenzig J

Clonal dynamics studied in cultured iPS cells reveal major growth imbalances within few weeks.
Stem Cell Research & Therapy. 2018 in press.

Timin AS, Muslimov AR, Lepik KV, Epifanovskaya OS, Shakirova AI, Mock U, Riecken K, Okilova MV, Sergeev VS, Afanasyev BV, Fehse B, Sukhorukov GB

Efficient gene editing via non-viral delivery of CRISPR-Cas9 system using polymeric and hybrid microcarriers.
Nanomedicine. 2018 Jan;14(1):97-108. doi: 10.1016/j.nano.2017.09.001. Epub 2017 Sep 14.

Giersch K, Bhadra OD, Volz T, Allweiss L, Riecken K, Fehse B, Lohse AW, Petersen J, Sureau C, Urban S, Dandri M, Lütgehetmann M.

Hepatitis delta Virus persists during liver regeneration and is amplified through cell division both in vitro and in vivo.
Gut. 2017 Dec 7. pii: gutjnl-2017-314713. doi:10.1136/gutjnl-2017-314713. [Epub ahead of print]

Chai C, Rivkin M, Berkovits L, Simerzin A, Zorde-Khvalevsky E, Rosenberg N, Klein S, Yaish D, Durst R, Shpitzen S, Udi S, Tam J, Heeren J, Worthmann A, Schramm C, Kluwe J, Ravid R, Hornstein E, Giladi H, Galun E.

Metabolic Circuit Involving Free Fatty Acids, microRNA 122, and Triglyceride Synthesis in Liver and Muscle Tissues
Gastroenterology. 2017 Aug 9. pii: S0016-5085(17)36024-9. doi:10.1053/j.gastro.2017.08.013. [Epub ahead of print]

Lanton T, Shriki A, Nechemia-Arbely Y, Abramovitch R, Levkovitch O, Adar R, Rosenberg N, Paldor M, Goldenberg D, Sonnenblick A, Peled A, Rose John S, Galun E, Axelrod JH

Interleukin 6-dependent genomic instability heralds accelerated carcinogenesis following liver regeneration on a background of chronic hepatitis
Hepatology. 2017 May;65(5):1600-1611. doi: 10.1002/hep.29004. Epub 2017 Mar 23

Mohme M, Maire CL, Riecken K, Zapf S, Aranyossy T, Westphal M, Lamszus K, Fehse B

Optical Barcoding for Single-Clone Tracking to Study Tumor Heterogeneity
Mol Ther. 2017 Mar 1;25(3):621-633. doi: 10.1016/j.ymthe.2016.12.014. Epub 2017 Jan 18